Fig. 4
From: In vivo imaging of free radicals produced by multivitamin-mineral supplements
Configuration and area imaged by EPR to monitor in vivo formation of CP●. Anaesthetized (isoflurane 2.2 %) mice were positioned for imaging in a 36 mm cavity of L-Band EPR-Spectrometer ELEXSYS E540 equipped with an automatic matching control system. The area for imaging (30 × 30 mm) was chosen as presented (blue circle with 3 black dots) to capture the digestive system. Acquisition of the spectra for CP● in the digestion system was performed twice (2 × 10 min). EPR instrument settings were: center field, g = 2.01; field sweep, 60 G; microwave power, 40 mW; magnetic field modulation, 1 GHz; modulation amplitude, 3.0 G; conversion time, 20.24 ms; detector time constant, 40.96 ms; image field of view, 25 mm; acquired angles, 31; gradient, 24 G cm−1