Fig. 4

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) effectively protected against mucosal inflammation and integrity. a Gross histological images of the small intestine after 5-fluorouracil treatment. Bar length = 100 μm. b Mucosal villi length and (c) crypt length were also measured. d Diamine oxidase activity (DAO) a measure of gut permeability and integrity was also assessed. Values are expressed as mean ± SD. N = 7 per experimental groups. *P < 0.05, **P < 0.01, ***P < 0.001. FO, Fish oil