Table 1 Mitochondrial respiration protocol reagents listed in order of titrations and the corresponding pathway controls and respiratory states
From: Neonatal diet impacts liver mitochondrial bioenergetics in piglets fed formula or human milk
Substrates | Abbreviations | Pathway control/ Respiratory state | Pathway to Q | Explanation |
|---|---|---|---|---|
Pyruvate (5 mM), Malate (2 mM), Glutamate (10 mM) | PMG | N/PMG(L) | CI | LEAK (L) respiration, no adenylates |
ADP (5 mM) | ADP | N/PMG(P) | CI | OXPHOS capacity (P) |
Succinate (10 mM) | SUC | NS/PMGS(P) | CI + CII | Complex I + II-linked (P) |
Oligomycin (0.2 μg/ml) | OMY | ROX | ROX | Inhibition of CV |
Antimycin A (12.5 μM) | AMA | ROX | ROX | Inhibition of CIII |