Table 2 Primers used for mitochondrial DNA copy number measurement in piglet ileum and liver
From: Neonatal diet impacts liver mitochondrial bioenergetics in piglets fed formula or human milk
Gene | Forward Primer 5′-3’ | Reverse Primer 5′-3’ | Amplicon (bp) | Use |
|---|---|---|---|---|
Cox1 | CTCTGGGCTTCATCTTCCTATTC | GAGGACATCCGTGTAGTCATTC | 477 | Standard Curve |
Nd1 | GCCTAGCAGTATACTCTATCCT | CGTATCGGAATCGTGGGTATG | 519 | Standard Curve |
Actb | CTGGCATTGTCATGGACTCT | GATCGAGTTGAAGGTGGTCTC | 496 | Standard Curve |
Cox1 | GTAGTCGCACACTTCCACTATG | TGAGTGTGTACCCGGAGAATA | 100 | Real-time PCR |
Nd1 | TTATCTCAACCCTAGCAGAAACC | AAAGGTCCGGCTGCATATT | 103 | Real-time PCR |
Actb | ACCTGACCGACTACCTCAT | GCAGAGCTTCTCCTTGATGT | 101 | Real-time PCR |