Table 3 Primers used for mitochondrial gene expression assays in piglet ileum and liver
From: Neonatal diet impacts liver mitochondrial bioenergetics in piglets fed formula or human milk
Gene | Forward Primer 5′- 3’ | Reverse Primer 5′- 3’ |
|---|---|---|
Cycs | CCAAACCTCCATGGTCTCTTT | TACTCCATCAGTGTCTCCTCTC |
Hspa9 | TCAACAGGAACACCACCATT | TCTCTCTCACCCTGACATACTT |
Nrf1 | CGATGGCACTGTCTCTCTTATC | CCATCAGCCACAGCAGAATA |
p53 | CCGAGTACTTGGATGACAGAAA | GTTGTAGTGGATGGTGGTACAG |
Pgc1α | CCCACAACTCCTCCTCATAAAG | TCACTGTACCGGGTCTTCT |
Pparβ | CATGAAGCTGGAGTACGAGAAG | AGCGAATGGCGTTGTAAGA |
Sco2 | AAGGACGAGGACCAGGATTA | TGCCGTAGTAGTCGGTGAA |
Tfam | GTGGAGGGAACTTCCTGATTC | TGACTTGGAGTTAGCTGTTCTT |
Trap1 | CAGCTTCGACCACCTCTTC | CCAGCAGCCCATCCTTATT |
Uqcr10 | GAGGTTGTACTCCCTGTTGTTT | CCTGGTTGATGTGTTCGTAGAT |
Rps16 | TGATCCTCGTCGCTGTGAATCCAA | ACGCTGATCATCACGATGGGCTTA |
Rpl27 | AAGTTCATGAAACCCGGGAAGGTG | TCGGTCTGAGGTGCCATCATCAAT |
18S | CCTGTAATTGGAATGAGTCCAATTT | ATACGCTATTGGAGCTGGAATTACC |