Fig. 3

The mobility of animals exposed to Bioact®180 is maintained over time. Worms (strain, N2) were placed on no compound control (circles), 0.2% DMSO (squares) or 1000 µg/mL Bioact®180 (triangles) supplemented NGM as L4s (at T = 0). The number of head-to-tail body bends were assessed for each condition with the average and standard error of the mean shown. On day 4, worms in control conditions (open circles; n = 18) had the same number of body bends per minute, BBPM, as those exposed to DMSO (open squares; n = 19) or Bioact®180 (open triangles; n = 15). When the worms are 8 days of age, the number of BBPM significantly decreases when worms are exposed to control conditions (closed circles; n = 18) and the DMSO vehicle control (closed squares; n = 18). In contrast, worms on 1000 µg/mL Bioact®180 (closed tringles; n = 10) show no change in BBPM, suggesting that the compound is providing some health benefit. p-value obtained by the 2-tailed 2-sample t-test, where *p < 0.05 and **p < 0.001. If no asterisks, there is no significant difference