Fig. 4

Muscle morphology is unchanged in Bioact®180 exposed worms compared to wild type. Worms (strain, RW1596) were placed on no compound control, 0.2% DMSO or 1000 µg/mL Bioact®180 supplemented NGM as L4s (at T = 0). The muscle morphology was observed via the myo-3p::GFP reporter for each condition on days 4, 8 and 15 and assessed as being linear or fragmented. (a) Representative image of a worm displaying (i) linear or (ii) fragmented muscle fibres. Scale bars, 100 µm. (b) Graph to show the percentage of worms in each category at the different time points. Black bars indicate linear muscle morphology while the grey bars are for those fibres which are fragmented. Data was from 4 independent biological replicates with more than 85 worms assessed on day 4 (control n = 88; DMSO n = 85; Bioact®180 n = 85) and more than 95 worms on day 8 (control n = 110; DMSO n = 95; Bioact®180 n = 93), and around 20 worms on day 15 (control n = 28; DMSO n = 19; Bioact®180 n = 24)