Fig. 5

Mitochondrial morphology of worms exposed to Bioact®180 have less degradation on day 8 of life. Worms (strain, SD1347) were placed on no compound control, 0.2% DMSO or 1000 µg/mL Bioact®180 supplemented NGM as L4s (at T = 0). The mitochondrial network of worms was assessed for linear, intermediate or fragmented morphology on day 4 and 8. (a) Representative image of a worm displaying a (i) linear or (ii) fragmented mitochondrial network. Scale bars, 100 µm. Graphs to show the mitochondrial morphology on (b) day 4 and (c) day 8. The classifications are as follows: linear (black bars) where the mitochondrial network has even linear lines; fragmented (white bars) is where the network has reduced significantly and is mostly broken; an intermediate classification (grey bars) is where the network is uneven and starting to fragment. The mitochondrial morphology was assessed over 2 independent experiments, with a total of n = 40 for day 4 control, day 4 Bioact®180 and day 8 Bioact®180; n = 38 for the DMSO control at day 4 and 8; n = 41 for control worms on day 8