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Table 2 Biospecimen processing supplies

From: Collection of biospecimens from parent-child dyads in a community garden-based nutrition intervention: protocol and feasibility

Item Name

Vendor

Catalog Number

Comment/ Description

Isopropanol (LCMS grade)

Fisher Scientific

A461-500

One bottle

Cryobabies Labels Laser Sheet, 1.5 – 2.0 mL Tubes

Fisher Scientific

50-998-639

One label for each planned cryovial

Thermo Scientific Nalgene Long-Term Storage 2 mL Cryogenic Tubes

Fisher Scientific

03-337-7D

One tube for each planned aliquot

Stool

 Sodium Butyrate (13C499%)

Cambridge Isotope Laboratories

CLM-1256-1

One container

 QIAamp DNA Stool Mini Kit

Fisher Scientific

51306

250 count

 Tris Hydrochloride

Sigma

108128460010

500g

 Triton X-100

Sigma

X100

 

 Mutanolysin (from Streptomyces globisporus ATCC21553)

Sigma

M9901-10KU

10K units

 Lysozyme (from chicken egg white)

Sigma

L6876

 

 0.1 mm Diamter Zirconia/Silica Beads

BioSpec Products

11079101z

1lb bottle

 Proteinase K

Qiagen

19131

2 mL

 InhibitEX

Qiagen

19593

140 mL

 Buffer AL

Qiagen

19075

264 mL

Urine

 Trimethylamine N-oxide (D9,98%)

Cambridge Isotope Laboratories

DLM-4779-PK

One container

Hair

 Cortisol Enzyme Immunoassay Kit 

Salimetrics

#1-3002

 

 Isopropanol (HPLC grade)

Fisher Scientific

BP2632-4

 

 Methanol (HPLC grade)

Fisher Scientific

A452-1

 

 Chrome/Steel Grinding Balls

Retsch

05-368-0032

2 per sample

 15 ml screw-cap polypropylene conical tube

Fisher Scientific

05-539-4

 

 1.5 ml round end microcentrifuge tube manufactured with reinforced plastic

Eppendorf

022363352

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