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Table 2 Antagonistic activities of LAB with different physicochemical treatments. Screening of antimicrobial activities of LAB after different treatment of cell free supernatants (CFS)

From: Antibiotic resistant microencapsulated probiotics synergistically preserved orange juice

Treatment

B. cereus

B. subtilis

Staphyl. aureus

E. coli

Shi. dysenteriae

Shi. sonnei

Saccharomyces cerevisiae

Aspergillus niger

C. albicans

LAB1 CSF (100 μl)

++++

++++

++++

++++

++++

++++

+++

+++

+++

 Neutralized by NaOH

+++

+++

++

+++

+++

++

+++

++

++

 Catalase

++

++

++

+++

++

++

++

++

+

 Protease K

+

-

+

+

+

++

-

+

+

 Heat 121 °C for 15 min

+++

+++

+++

+++

+++

+++

++

++

++

LAB2 CSF (100 μl)

++

+++

++

++

+

++

+

+

+

 Neutralized by NaOH

++

+++

++

++

+

++

+

+

+

 Catalase

++

++

++

++

+

++

+

+

+

 Protease K

+

+

+

+

-

-

-

-

+

 Heat 121 °C for 15 min

+

+

+

+

-

-

-

-

+

LAB3 CSF (100 μl)

++++

+++

+++

+++

+++

++++

++

++

++

 Neutralized by NaOH

+++

+++

+++

+++

++

+++

+/-

+

+

 Catalase

++

++

++

++

++

++

+

+

++

 Protease K

+

-

+

+

+

++

++

+/-

+

 Heat 121 °C for 15 min

+++

++

++

+++

++

++

++

+

++

LAB4 CSF (100 μl)

++

+

+++

+++

++

++

++

++

+

 Neutralized by NaOH

+

+

++

++

++

++

++

++

+

 Catalase

++

+

+++

++

++

++

++

++

+

 Protease K

+

+

-

-

-

+

+

-

+

 Heat 121 °C for 15 min

++

+

++

++

++

++

++

++

+

  1. -, No effect; +, 10.1-15 in mm; ++, 15.1-20 in mm; +++, 20.1-25 in mm; ++++, >25.1 in mm