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Table 1 Participant inclusion and exclusion criteria

From: The Break-Fast study protocol: a single arm pre-post study to measure the effect of a protein-rich breakfast on autophagic flux in fasting healthy individuals

Inclusion criteria

 Aged between 20 and 50 years of age

 BMI between 18.5 and 29.9 kg/m2

Exclusion criteria

 Co-morbidities that are likely to change the activity of the lysosomal system, such as cancer, diabetes, cardiovascular disease/conditions (stroke, heart attack, high blood pressure), major psychiatric disorders (schizophrenia, addiction, eating disorders, major depressive disorder), neurological disorders, dementia.

 Any lifestyle (e.g. physical activity, dietary habits) and/or change in lifestyle deemed likely to affect the results by the study principal investigator.

 Taking any medications that might change autophagic activity; including, but not restricted to, medications that change body composition or metabolism (e.g. medications used to lower blood glucose, antidiabetic medications), anti-inflammatory medications, medications that change brain function (e.g. antidepressant medications, mood stabilisers, lithium), medications used in the treatment of cancer or cardiovascular disorders.

 Female participants taking hormonal contraceptive will not be excluded from the study, if they have a regular menstrual cycle, they will be asked to attend the study appointment between day 1 and day 10 of their cycle.

 Current alcohol and/or substance use disorder.

 Lactose intolerance (except if managed by medication e.g. Lacteeze).

 Vegan.

 Allergies preventing consumption of the study product.

 Current smoker.

 Pregnant or breastfeeding women or women planning a pregnancy.

 Women who have been through menopause.

 Anyone who is unable to comprehend the study protocol (i.e. due to English language or cognitive difficulties).