Table 1 Primer sequences for studied genes
Target | Primer sequences |
|---|---|
CD39 | F5’-AGTGATTCCAAGGTCCCAGCA-3’ R5’-CTGGCACCCTGGAAGTCAAAG-3’ |
CD47 | F5’-GCCTTGTCCCTATTGTGGCTT-3’ R5’-CTTGGTTTGTGACTGCCCCAT-3’ |
CD73 | F5’-CCAGTCCACTGGAGAGTTCCT-3’ R5’-ACTCGACACTTGGTGCAAAGA-3’ |
CD80 | F5’-AAGAAGTGGCAACGCTGTCCT-3’ R5’-CGGTTCTTGTACTCGGGCCAT-3’ |
PD-L1 | F5’-CTGCAGGGCATTCCAGAAAGA-3’ R5’- TGCGTTCAGCAAATGCCAGTA-3’ |
B7-H3 (CD276) | F5’-TGACCACATCACCACCCTCTT-3’ R5’-AGCAGGGCTGAACATGATCAC-3’ |
B7-H4 | F5’-ATTTCAGCCTTCAGCATGCCG-3’ R5’-GGTCAACTTGGGATGCCCAGA-3’ |
GAPDH | F5’- CAAGATCATCAGCAATGCCTCC-3’ R 5’-GCCATCACGCCACAGTTTCC − 3’ |